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ITSI Protein Isolation Kit:蛋白質純化試劑組
The ToPI-PAGE kit contains a set of optimized buffers, consumables, and standard operating procedure that allow the isolation of total proteins from biological and clinical samples with no protein breakdown. ToPI-PAGE is optimized for isolation of total proteins from cell lines (K-0011-C) and mammalian tissue (K-0011-T) prior to 2-Dimensional polyacrylamide gel electrophoresis (2D-PAGE). In most cases it allows a smooth transition from the protein isolation step to the downstream analytical processes without the need to perform a buffer exchange. The standard ToPI kit will allow quick and convenient isolation of proteins from 10, 20 and 30 samples.
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ITSI Protein Fraction & Enrichment Kit:細胞核細胞質細胞膜-蛋白質分相純化試劑組
可有效針對 細胞核nuclear, 細胞質cytosol 及 細胞膜membrane regions 中蛋白質進行分相純化,不需高速離心即可完成;
後續可應用於mass spectrometry, SDS-PAGE (Fig 1), 2D-PAGE, 2D-DIGE (Fig 2) 及 western blotting。 -
ITSI Protein Precipitation Kit:蛋白質沈澱試劑組
簡單方便且可重複性地有效沉澱 total protein,達90%以上recovery。
沉澱後之 protein 後續可適用於1D-PAGE, 2D-PAGE, 2D-DIGE及western analysis。 -
ITSI Albumin Removal Kit:去除白蛋白試劑組
人類血中的白蛋白 (Albumin, 66 kDa)含量豐富,高達血漿/血清中總蛋白的65%以上。因高量白蛋白會遮蔽並影響其他微量蛋白質的偵測,多數實驗前須先將白蛋白移除以降低其干擾 。