產品特色：

• Extreme thermal stability: withstands three months at 25 ºC with no impact on efficiency
• Aptamer-based hot start system reduces non-specific amplification and primer dimers
• Overcomes inhibition in crude extracts and environmental samples
• Amplifies up to 4 kb from genomic DNA, and GC-rich targets with up to 70% GC-content
• License-free for commercial or diagnostic use

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產品使用說明：

詳見原廠產品使用手冊↓

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實驗參考數據：

• Figure-1：高效率且結果具高度線性↓

                   Ara h2 gene fragment                                           E = 98.2%, R² = 1.000
 
Exceptional efficiency & linearity is demonstrated using Sahara Hot Start PCR Master Mix to amplify a 157 bp gene fragment on an Open qPCR instrument in this 5-point standard curve.

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• Figure-2：適用廣泛檢體↓

       Amplification of 
    42 – 67% GC Content        Amplification up to 4 kb
 

Left: HPRT (42% GC), Ara h2 (51% GC), hActin3 (62% GC), and Salmon Sperm (67% GC) targets, ranging from 77 – 172 bp, were amplified on an Open qPCR instrument with Sahara Hot Start. All targets resolved well.

Right: Lambda phage targets of 500 bp, 1000 bp, 1978 bp, and 4003 bp show consistent amplification using Sahara Hot Start.

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• Figure-3：酵素穩定度極佳↓

             Ara h2 gene fragment

Sahara Hot Start PCR Master Mix was stored at 50 ºC, and a singleplex qPCR reaction was run daily on the Open qPCR instrument. No difference in C_q is observed throughout the trial.

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• Figure-4：速度及效率兼備之Hot Start↓

A challenging salmon sperm assay with 67% GC content was run with and without the Sahara Hot Start aptamer. The aptamer hot start successfully prevents the non-specific products seen in the non-hot start assay.

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規格內容：

Specification of Sahara Hot Start PCR Master Mix

Concentration	2X
Hot Start Mechanism	Aptamer
GC Content	40 – 70%
Reaction Speed	Fast
Max Amplicon Length	4 kb
Exonuclease Activity	5' → 3'
Supported Probes	TaqMan/Hydrolysis, Molecular Beacon, Scorpions
Supported Templates	Genomic DNA, cDNA, Plasmid DNA
Recommended Reaction Volume	10 – 50 µL

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Order information of Sahara Hot Start PCR Master Mix

Cat. No.	Quantity
#R02151S	2 * 1ml
#R02151M	5 * 1ml
#R02151L	2 * 5ml
#R02151XL	5 * 5ml
#R02151B	1 * 50ml
#R02151BL	5 * 50ml

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訂購與聯絡方式：

歡迎隨時聯繫產品專員進一步了解產品細節及詢價
亦可至線上諮詢留言處留下您的需求及聯絡方式
02-2771-0369 #26
ken.lin@pan-asia-bio.com.tw

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更新日期：

2022年8月18日