• Dynamic Range 超廣且結果具高度線性↓

  

Dynamic range of 10¹⁰ is demonstrated while maintaining excellent efficiency and linearity using Sahara Multiplex qPCR Master Mix to amplify a 157 bp gene fragment on an Open qPCR instrument.

• 任何檢體之實驗結果皆具高度一致性↓
                Enterococcus gDNA                            mGAPDH gene fragmen
               E = 101.8%, R² = 0.999t                      E = 99.4%, R² = 0.999
  

        

Four targets were amplified simultaneously using Sahara Multiplex qPCR Master Mix on a Bio-Rad CFX Touch instrument. 10-fold dilutions of all targets achieved excellent linearity & efficiency.

• 絕佳穩定度↓

Sahara Multiplex qPCR Master Mix was stored at 50 ºC, and a fourplex reactions were run daily on Bio-Rad CFX96 Touch instrument. No difference in C_q is observed throughout the trial.

• 速度超快效率極佳↓

 

A fast 19 minute protocol was run on an Open qPCR instrument, obtaining excellent linearity & efficiency. Protocol: Initial denaturing 30s @ 95 ºC, Cycle 30x: 3s @ 95 ºC, 14s @ 60 ºC, 5 C ºC/s ramp times.